After installing all prerequisite dependencies and setting up a XenApp 6.5 Web Interface 5.4 on IIS 7.5, I was getting the error "401.3 - Unauthorized: Access is denied due to an ACL set on the requested resource" whenever I tried to access the web interface front end using the default IUSR account for anonymous authentication. I tried every combination of troubleshooting I could think of: looked through IIS logs, changed the Anonymous Authentication credentials to use the Application pool … [Read more...]